nasba isothermal technique: a novel tool for mycobacterium tuberculosis diagnosis

نویسندگان

امیر قائمی

a ghaemi assistant professor, , infectious diseasesمرکز تحقیقات عفونی و گروه میکروب شناسی، دانشگاه علوم پزشکی گلستان پوریا گیل

p gill گروه نانوبیوتکنولوژی، دانشکده فناوریهای نوین دانشگاه تربیت مدرس، عبدالوهاب مرادی

a v moradi مرکز تحقیقات عفونی و گروه میکروبشناسی، دانشگاه علوم پزشکی گلستان علیجان تبرایی

a tabaraei شناسی، دانشگاه علوم پزشکی گلستان مرکز تحقیقات عفونی و گروه میکروب

چکیده

background and objectives: rapid, accurate and cost effective diagnosis of infectious and non infectious diseases is an essential step for treatment process. nowadays, in line with scientific progression in molecular biology, genetics and biochemistry which are based on biotechnology and genetic engineering aspects, new branch of medicine entitled molecular medicine is being derived. it can be helpful in three areas of diagnosis, prophylaxis and treatment. this new branch is going to identify further complexity of diseases and to present efficient solutions for growing health criteria. therefore, updating and being familiar with the new procedures related to diagnosis, prophylaxis and therapy are necessary for our society. in this paper, we are trying to introduce nasba technology which has a high potential, at genome level, in recognizing specific characteristic and unique genetic markers of microorganisms. this technology has numerous benefits for easy detection of infectious diseases such as tuberculosis. furthermore, we review the methods of tuberculosis detection.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

NASBA Isothermal Technique: a Novel Tool for Mycobacterium tuberculosis Diagnosis

Background and Objectives: rapid, accurate and cost effective diagnosis of infectious and non infectious diseases is an essential step for treatment process. Nowadays, in Line with scientific progression in molecular biology, genetics and biochemistry which are based on biotechnology and genetic engineering aspects, new branch of medicine entitled molecular medicine is being derived. It can be ...

متن کامل

Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.

IS986 of Mycobacterium tuberculosis belongs to the IS3-like family of insertion sequences, and it has previously been shown to be present in multiple copies in the chromosome of M. tuberculosis. In this study we investigated the value of a IS986-based DNA probe in the diagnosis and epidemiology of tuberculosis. IS986 was found only in species belonging to the M. tuberculosis complex. Independen...

متن کامل

CYP141 gene: A New approach for Mycobacterium Tuberculosis Identification

In summary, in order to control tuberculosis, we require a rapid diagnosis of TB patients, who act as the source of disease; and if remain untreated, these patients can spread the disease throughout the population. Therefore, we need to introduce the new approach for quick and accurate identification of Mycobacterium tuberculosis, and CYP141 gene is a new approach for rapid and accura...

متن کامل

Diagnosis of Tuberculosis: Novel Laboratory Methods

Despite advances in medical sciences in recent years, tuberculosis remains a worldwide health problem. Although there are many laboratory methods to accelerate the diagnosis, a delay in the definitive diagnosis of the disease is still a major clinical problem. Due to the slow growth of the causative agent, (Mycobacterium tuberculosis), isolation, identification, and other clinically important m...

متن کامل

NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection.

Isothermal nucleic acid amplification of target RNA or DNA sequences is accomplished by the simultaneous enzymatic activity of AMV reverse transcriptase, T7 RNA polymerase and RNase H. Amplification factors of the nucleic acid sequence based amplification (NASBA) method range from 2 x 10(6) to 5 x 10(7) after 2.5 h incubation at 41 degrees C. During NASBA there is a major accumulation of specif...

متن کامل

Diagnosis of Mycobacterium tuberculosis

Tuberculosis (TB), caused by the intracellular bacterium, Mycobacterium tuberculosis (Mtb), has been a major health concern since it plagued ancient Egypt 5 thousand years ago. TB infects 9 million people every year, most of them children (especially in endemic areas), and it leads to approximately 2 million deaths annually (World Health Organization [WHO], 2008; Kabra & Lodha, 2004; Marais & P...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical laboratory journal

جلد ۴، شماره ۱، صفحات ۰-۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023